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However, those chromatographic modes are inherently hamiş compatible with MS and therefore there is a need for finding novel volatile mobile phase buffer systems. Very interesting recent work by Mary Wirth should also be mentioned here, namely an MS-compatible alternative to HIC called native reversed-phase liquid chromatography (nRPLC).
We saw that friction related effects were more important for small molecule separations whilst pressure effects were much more significant for protein separations. Insulin was an attractive example bey we could clearly see the decrease of retention in constant inlet pressure mode and the increase of retention in variable inlet pressure mode when increasing the flow rate.
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A huge impact was observed even in gradient elution mode, which was not expected. What prompted you to specifically investigate operating pressure bey a separation variable? How is what you have discovered different from what others have thought previously?
Mk Usmaan is an avid AI enthusiast who studies and writes about the latest developments in artificial intelligence. Bey an aspiring computer scientist, he is fascinated by neural networks, machine learning, and how AI technology is rapidly evolving.
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On the other hand, this extreme effect could cause serious issues when transferring previously developed conventional methods to UHPLC method.